01
Harvesting the Whole Blood into T-LAB PRP Tube

The whole blood is harvested into T-LAB PRP tube from the patient’s arm using phlebotomy set of T-LAB PRP Kit. Each T-LAB PRP Tube is 10ml volume including 1ml of anticoagulant. Both tubes stop when vacuum is finished. So, you have a total 18ml of whole blood. After harvesting the whole blood, gently shake both tubes to permeate the anticoagulant into whole blood in the tubes.

02
Centrifugation

Tubes shall be put accordingly in order to achieve the centrifuging balance. No special centrifuging device is provided or recommended. The most important subject for centrifuging is to perform 830 G for 8 minutes according to instructions for use. Swing rotor centrifugation devices are recommended for more convenient results. 

03
Harvesting the PRP

This section is the crucial part to obtain the platelet rich plasma. Please do not shake or move the tubes upside down after centrifugation process. After centrifugation you shall see 3 layers in the tube. It means centrifugation process is done properly.

Understanding the PRP Layer

Centrifugation process makes the cell layers separated each other according to the molecular weight of the cells. RBC (Red Blood Cell) layer is formed at the bottom of the tube as RBCs are bigger cells in comparison to other component of the whole blood. Right above the RBC layer, there is buffy coat layer with a very small volume (<%1 of the whole blood vol.) where the possible clump formation occurs. Upper layer is yellowish colored. This layer includes PRP and PPP layers together. But those layers can not be recognised with the naked eye. PRP is defined as 2ml volume right above the buffy coat layer.

Harvesting the PRP

Long needle is attached to 5ml injector and put vertically into the tube. The edge of the needle is placed right on the Buffy coat layer and PRP is collected with tornado movement of the needle and injector. 2ml of PRP shall be collected. It is also recommended to collect small amount of RBCs along with Buffy Coat layer and PRP. This is important for the Cell-Cell interaction during the Re-suspension process.

04
Re-Suspension Process

The PRP collected into 5ml injector is carried to T-LAB Re-Suspension Tube. Re-suspension Tube is plain and sterile. This tube is 5ml volume and total 4 ml PRP collected from T-LAB PRP Tubes (2ml each as recommended) are placed in this tube. After carrying the PRP into T-LAB Re-suspension tube, gently shake the tube for about 30 seconds to 1 minute to avoid possible clumped cells. This process supports the homogenisation of the cells in the plasma milieu. Attach 21G needle to 1ml injector and collect the PRP inside from the T-LAB Re-suspension tube. Change the needle to 30G and you     are ready to apply the PRP.